壳聚糖/纳米铜复合微球的制备及其对刚果红染料的脱色性能     

李瑞明  李瑾  钟莲  朱巧雅 《中国海洋大学学报(自然科学版)》2022,52(2):77-86

以壳聚糖和CuCl₂·2H₂O为原料,分别采用直接负载法和吸附法成功制备了壳聚糖/纳米铜复合微球,运用傅里叶变换红外光谱(FT-IR)、X-射线衍射(XDR)和扫描电镜(SEM)对催化剂结构进行了表征。以刚果红(CR)染料为目标污染物,评价了催化剂的制备方法、制备条件及氢转移催化反应条件对其催化性能的影响。结果表明,由直接负载法(CS/CuNPs)和吸附法(CS/CuNPs-X)制备的壳聚糖/纳米铜复合微球在CR染料浓度为100 mg/L,催化剂投加量为0.05 g,供氢体浓度为0.05 mol/L的氢转移催化反应条件下,催化反应进行180和60 min时CR脱色率可分别达95.8%和96.8%。CS/CuNPs-X比CS/CuNPs表现了更高的对CR氢转移催化反应的催化速率。两种催化剂催化还原CR染料的反应均符合准一级动力学模型。在连续循环使用10次后,CS/CuNPs和CS/CuNPs-X对CR的脱色率分别为93.9%和89.4%,表明催化剂具有良好的重复使用性能。  相似文献   

6种海洋致病性弧菌36kDa外膜蛋白特性分析   总被引：3，自引：0，他引：3  

唐小千  战文斌  周丽  绳秀珍 《中国海洋大学学报(自然科学版)》2009,39(2)

研究本着筛选和制备弧菌共同外膜蛋白亚单位疫苗,用十二烷基肌氨酸钠抽提并结合超速离心的方法提取了鳗弧菌(Vibrio anguillarum)、鱼肠道弧菌(V.ichthyoenteri)、溶藻弧菌(V.alginolyticus)、副溶血弧菌(V.parahaemolyticus)、哈维氏弧菌(V.harveyi)和创伤弧菌(V.vulnificus)6种海洋致病性弧菌的主要外膜蛋白, SDS-PAGE图谱比较发现这6种弧菌都存在36 kDa的外膜蛋白条带.通过电泳洗脱分离纯化这6种弧菌的36 kDa外膜蛋白,并制备了鳗弧菌菌株SJ060621的36 kDa外膜蛋白多抗.ELISA和Western-blot印迹结果显示,仅鳗弧菌SJ060621、S010610-1和哈维氏弧菌的36 kDa外膜蛋白存在免疫交叉反应;双向电泳分析了鳗弧菌SJ060621、S010610-1、哈维氏弧菌和溶藻弧菌的36 kDa外膜蛋白,2-DE图谱显示出鳗弧菌SJ060621和S010610-1均由3种等电点相近的蛋白组成,存在很高的同源性,2株鳗弧菌与溶藻弧菌和哈维氏弧菌在蛋白质组成数量和等电点上都存在较大的差异,结果说明通过主要外膜蛋白的分子量和免疫特性研究,可为筛选不同弧菌共同外膜蛋白的亚单位疫苗提供有利参考.  相似文献   

赤魟软骨血管生成抑制因子的分离纯化   总被引：2，自引：0，他引：2       下载免费PDF全文

余新威  钱晓  王婕妤  罗红宇  吴常文 《海洋学报》2008,30(1):113-118

确定了制备高纯度赤魟软骨血管生成抑制因子的纯化工艺,并通过胶原酶模型、CAM模型验证其生物学活性。正交实验确定了赤魟软骨组织抽提的最佳条件,抽提产率可达0.84%。丙酮分级沉淀对抽提产物进行粗分离,25~35%的丙酮分级产物的CAM血管生成抑制率达85%。优化离子交换层析、凝胶过滤、反相层析的条件,可进一步有效的获得高纯度的赤魟软骨血管生成抑制因子-I(DCAIF-I)。SDS-PAGE-考马斯亮蓝染色显示为一条带,分子量为62 kDa。活性验证结果表明,DCAIF-I对胶原酶具有一定的抑制活性,抑制率为36.3%,对CAM血管生成的抑制活性具有一定的剂量依赖关系。  相似文献   

Preparation and Charaterization of Self-assembled Nanoparticles Based on Linolenic-acid Modified Chitosan     

Desai Kashappa Goud H.  Park Hyun-Jin 《中国海洋大学学报(英文版)》2005,4(3):234-239

1 IntroductionHydrophobically modified water-soluble polymers,also known as hydrophobized polymers, are thosemodified by hydrophobic groups such as alkyl,arolkyl, and deoxycholic acid. In water, they canself-aggregate due to their intro- and/or intermolecularhydrophobic interaction. By this process, unique hy-drogel nanoparticles with hydrophobic core and hy-drophilic shell can be prepared, which is a new ap-proach to preparing the monodispersed hydrogelnanoparticles (Whitesides et al., 1991; …  相似文献   

膜生物反应器中膜污染机理及其防治     

宋志伟  杨帆  任南琪 《吉林大学学报(地球科学版)》2008,38(4)

通过向一体式膜生物反应器中分别投加聚合铝和粉煤灰改变料液性质,来预防膜污染和提高膜生物反应器对总磷的去除效率,并通过X射线衍射和红外光谱实验分析活性污泥性质的变化,利用扫描电镜分析中空纤维膜表观结构的变化情况,探讨防治膜污染的机理.实验结果表明:聚合铝的投加改变了活性污泥的性质和生物膜的表观结构,可有效地减缓膜污染,且除磷效率达85%以上;而投加粉煤灰并没有明显效果.  相似文献   

Researches on the Internal Molecular Weight Uniformity of Chitosan Biomaterials     

LANG Xuqian  LI Yang  SUN Guohui  ZHOU Zhongzheng  LI Jing  FENG Chao  KONG Ming  LIU Ya  CHENG Xiaojie  CHEN Xiguang 《中国海洋大学学报(英文版)》2020,19(2):459-465

Uniform molecular weight(Mw)chitosan(CS)is highly demanded in medical biomaterial industry.This present article described heterogeneous degradation of CS in aqueous HCl/ethanol solution,in which progress uniform Mw CS was successfully prepared.The Mw distribution of CS was measured by gel permeation chromatography(GPC)analysis.Moreover,the structure and properties of degraded CS were characterized by Fourier transform infrared spectroscopy(FT-IR),nuclear magnetic resonance spectroscopy(^1H NMR),X-ray diffraction(XRD)and thermogravimetric(TG)analysis.In addition,the biocompatibility of degraded CS was also assessed by hemolysis rate(HR)measurement.The Mw of CS dramatically decreased from 246 KDa to 76 k Da at the initial 30 min,and stabilized at 18 kDa after 24 h.GPC analysis results showed that the degraded CS molecular become homogenization.FT-IR and 1 H NMR analysis confirmed the basic structure of CS molecular backbone was not destroyed during this progress.Besides,the water solubility of CS was not significantly influenced by this reaction.Moreover,the XRD analysis revealed that crystallinity of degraded CS increased from 70.32% to 99.25%with time.The TG analysis showed improved thermal stability of degraded CS.HR measurement demonstrated the degraded CS possessed excellent biocompatibility.This simple and efficient heterogeneous degradation would open up a new route to produce uniform Mw CS.  相似文献   

胞外聚合物对膜生物反应器运行性能的影响   总被引：3，自引：1，他引：3  

张凤君  王顺义  邓锡斌  赵芝清  沙娜  王鹤 《吉林大学学报(地球科学版)》2006,(Z1)

膜生物反应器处理污水过程中,依据胞外聚合物(EPS)、蛋白质、多糖、粘度等参数随时间的变化趋势以及对膜通量的影响,求出上述参数及蛋白质/多糖的比值与膜通量间的相关系数分别为0.28、0.57、0.38、0.14、0.70。结果表明:蛋白质/多糖的比值与膜通量的衰减速度两者相关系数最大。  相似文献   

桩网复合地基桩土应力比的确定     

陈娟  吴西臣 《水文地质工程地质》2007,34(3):116-119

实践证明,桩网复合地基同时具备竖向增强体复合地基与水平向增强体复合地基的加固优点,能很好地提高地基土体承载力,减小不均匀沉降,特别是桥头过渡段地基处理中能有效控制“桥头跳车”现象的产生。本文主要对桩网复合地基的加固机理进行分析,并结合自然平衡拱理论和加筋垫层拉膜效应理论推导出路堤下桩网复合地基桩土应力比计算公式,同时分析了各设计参数变化对桩土应力比的变化规律。工程实体试验表明,本文所推导的桩土应力比计算公式具有较好的适用性。  相似文献   

电镀金刚石钻头镀前超声清洗机理及工艺研究   总被引：4，自引：0，他引：4  

刘晓东  霍宇翔  李子章 《地质与勘探》2006,42(1):89-92

电镀金刚石钻头镀前清洗是钻头生产的关键制造工艺.超声清洗作为一种精清洗技术,在不少领域获得了成功.本文阐述了超声波对金刚石钻头镀前清洗工艺的适应性,分析了电镀金刚石钻头中的主要清洗对象及超声清洗机理,对超声清洗中的各项工艺参数及工艺流程进行了研究,说明了超声清洗的经济适用性及可操作性.  相似文献   

Selenium contamination in the Colorado River Basin, western USA： Remediation by leaching and nanof＇dtration membranes     

YousifK. Kharaka Evangelos G. Kakouros James J. Thordsen David L. Naftz 《中国地球化学学报》2006,25(B08):100-101

Selenium （Se） is an essential micronutrient to biota, but can become a potent toxicant at elevated concentrations. The natural sources and chemical properties of Se species make the boundary between deficiency and toxicity narrow for some biota, with both phenomena common around the globe. Large areas of farmland in the Colorado River Basin （CRB） generate salinized drainage water with Se concentrations much higher than 5 μg/L, the U.S. Environmental Protection Agency chronic water-quality criterion for the protection of aquatic life. We have carried out detailed field and laboratory studies to investigate Se geochemistry and remediation in two of these areas： the Middle Green River Basin, Utah and the Salton Sea Basin, California, located respectively in the Upper and Lower CRB. Results from these and other studies show that approximately 90% of the dissolved Se in the Colorado River and its tributaries originally is derived from the Upper Cretaceous Mancos Shale and equivalent pyritic marine units that outcrop in the Upper CRB. Selenium is mobilized commonly by biogeochemical oxidation of this pyritic shale and is concentrated mainly as selenate （SeO4^2-） in soils and agricultural drainage water of dry climates by evaporation. Minor （0%-5%） amounts of Se are present as the selenite species （HSeO3^-） and （SEO3^2-）, but these species and the more reduced species, elemental Se （SeO） and selenide （Se^2-）, have much lower solubility and/or have high sorptive affinity towards organic matter, clay minerals and iron oxyhydroxides. The concentration of dissolved Se （-2.5 μg/L） and salinity in the Lower Colorado River water are among the highest of the world major rivers. Because of low precipitation （7 cm/a） and extreme evapotranspiration （-1.8 m/a） rates in the Salton Sea Basin, California, Se values in irrigation water imported from the Colorado River increase to 〉300 μg/L in drainage wastewater. Removal of Se from contaminated wastewater by nanofiltration membranes was demonstrated in laboratory and pilot-scale field experiments.  相似文献